An innovative coating technique for light and electron microscopic autoradiography.
نویسندگان
چکیده
منابع مشابه
Electron Microscopic Autoradiography
An improved method has been developed for the preparation of autoradiographs for electron optical study. The refinement lies principally in the routine production of uniformly thin layers of photographic emulsion over the tracer-labeled cell sections. This is accomplished by means of a centrifugal spreading mechanism. Maize root tips grown in the presence of H(3)-thymidine were examined electro...
متن کاملElectron Microscopic Autoradiography
Iodide is concentrated from the blood by the thyroid gland; after oxidation it is incorporated in tyrosyl residues of the thyroglobulin molecule. Thus the halogen is stored as a component of thyroglobulin in the colloid of the thyroid follicle. Secretion of thyroid hormone is considered to require proteolysis of the thyroglobulin and release of iodinated thyronines from the base of the acinar c...
متن کاملQuantitative Electron Microscopic Autoradiography
Quantitative electron microscopic autoradiography was used for comparing the binding of labeled somatostatin-14 (S-14) and somatostatin-28 (S-28) to islet cells. Monolayer cultures of rat islet cells were incubated with ['251-Tyr"jS-14 (S-14) or [125I-Leu8, D-Trp22, TyreJS-28 (S-281) in the presence or absence of excess unlabeled peptides. Autoradiographic grains (ARG) associated with individua...
متن کاملLight microscopic and electron microscopic histopathology of an iris microhaemangioma.
A patient who had been observed to have an iris microhaemangioma (capillary haemangioma), confirmed on fluorescein iris angiography, came to cataract surgery. The lesion was excised at the time of surgery and submitted to light and electron microscopic study. It had the features of a hamartoma of the capillary haemangioma type, with its characteristics being specific for vessels seen in iris ti...
متن کاملin the Angular Region of the Hamster: Light and Electron Microscopic Autoradiography
The distribution of S-sulfate and H-glucosamine in the angular region of the hamster was studied by light and electron microscopic autoradiography following intraperitoneal injection of these compounds to hamsters. Exposed silver grains of S-sulfate were concentrated in the trabecular meshwork, sclera, and cornea, and grains of H-glucosamine were localized in the trabecular region. The radioact...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1992
ISSN: 0022-1554,1551-5044
DOI: 10.1177/40.6.1588033